Research

Automatic Tracking and Motility Analysis of Human Sperm in Time-Lapse Images.

We present a fully automated multi-sperm tracking algorithm. It has the demonstrated capability to detect and track simultaneously hundreds of sperm cells in recorded videos while accurately measuring motility parameters over time and with minimal operator intervention. Algorithms of this kind may help in associating dynamic swimming parameters of human sperm cells with fertility and fertilization rates. Specifically, we offer an image processing method, based…


Cryopreservation in Eukaryotes – Chapter 11

Quality Control Factors Influencing the Successful and Reliable Implementation of Oocyte and Embryo Vitrification

Clinical vitrification evolved slowly, with interests and acceptance being commercially driven by the development of unique devices, safer solutions, and the misconception that ultra‐rapid cooling in an “open” system was a necessity to optimizing vitrification success. Furthermore, the dogma surrounding the importance of cooling rates has led to unsafe practices subject to excessive technical variation and risky modifications to create closed‐storage devices. The aim of this chapter is…


Elective Single Embryo Transfer Criteria

Elective single embryo transfer criteria should be applied to frozen embryo transfer cycles

ASRM Guidelines for the number of fresh embryos to transfer were developed initially in 2008 and then updated in 2013. The guidelines were developed to address the increasing number of twins and high order multiples that IVF programs were reporting to SART. They recommended that patients <35 years old have elective single embryo transfer (eSET) unless they had two or more previously failed IVF cycles,…


Single Center Validation of Routine Blastocyst Biopsy Implementation – JARG August 2016

Preimplantation Genetic Screening Saves Time, Heartache in Advanced Maternal Age IVF Treatment

Women in their 30s and 40s who are trying to become pregnant using in vitro fertilization (IVF) benefit greatly from preimplantation genetic screening (PGS), according to a new study by the Southern California Center for Reproductive Medicine, in conjunction with Ovation Fertility™. By incorporating PGS into the IVF process, the study concluded that women of advanced maternal age experienced higher live birth rates per egg…


Annals of Clinical and Laboratory Research 2015

Euploidy Predictability of Human Blastocyst Inner Cell Mass and Trophectoderm Grading

The selection of viable, potentially normal, embryos is vital to successful embryo transfers. Subjective morphology grading of blastocysts has been the most accepted and reliable standard to judge embryo quality. However, good to excellent embryo development alone does not necessarily insure euploidy. Our objective was to determine if independent quality grades for blastocyst inner cell mass (ICM) and trophectoderm (TE) can predict early embryo euploidy. Secondly, we aimed to develop a…


Validation of microSecure vitrification – Cryobiology October 2015

Validation of microSecure vitrification (μS-VTF) for the effective cryopreservation of human embryos and oocytes.

A novel, aseptic closed system vitrification (VTF) technique for the cryopreservation of embryos and oocytes has been developed and clinically validated in this study. It combines the practicality of embryo-containing sterile flexipettes stored safely and securely with 0.3 ml CBS™ embryo straws possessing weld seals. The cooling and warming rates of this double container system were determined using a data logger. Upon direct plunging into…


Journal of Fertilization – March 2016

The Historic Development and Incorporation of Four Assisted Reproductive Technologies Shaping Today’s IVF Industry

The concept of assisted reproductive technologies was most notably derived from the late 19th Century experiments of Sir Walter Heape who successfully transplanted rabbit embryos. Interestingly, it was not until the late 1940’s and 1950’s that renewed interests in rabbit embryo transfer and cryobiology occurred. The history behind developing effective procedures can be fascinating, though few could be more accidental than Dr. Chris Polge’s discovery…