Authors: Carrie Bedient, Ankita Raman, Forest Garner, Martha Aguirre, Chelsea Morrison, Shyni Thomas, Anika Bill, Bruce Shapiro
Objective: Assess relationships, if any, between embryo ploidy and the mean diameter of the follicle from which the oocyte originated.
Design: IRB-approved prospective observational cohort study.
Materials and Methods: Patients underwent routine ovarian stimulation with exogenous gonadotropins. During oocyte collection, follicle diameters were sonographically measured on two perpendicular axes, and the mean was calculated for each follicle.Embryos were group-cultured to the blastocyst stage according to mean follicle diameter (≤9.5, 10-12.5, 13-15.5, 16-18.5, 19-21.5, 22-24.5, 25-27.5, and ≥28mm). Next-generation sequencing (NGS) followed trophectodermbiopsy. Chi-square analysis compared aneuploidy rates among follicle diameter groups. Stepwise logistic regression was used to identify significant predictors of aneuploidy. P<0.05 was considered significant.
Results:There were 318 blastocysts derived from measured follicles that had definitive NGS results (normal or abnormal), while 4 embryos with no test result were excluded. Of the 318 included blastocysts, 169 had normal (euploid) copy numbers and 149 had abnormal (aneuploid) copy numbers.Chi-square analysis found no relationship between aneuploidy and follicle size group (P=0.46). Among variables available for logistic regression, only patient age was retained in the logistic model (P=0.0020), while mean follicle diameter and blastocyst morphology parameters (blastocyst expansion, ICM, and trophectoderm quality)were not significant.
Conclusions: Among blastocysts selected for biopsy, the diameter of the originating follicle was not a significant predictor of embryo ploidy.
|Mean follicle diameter||Blastocysts||Euploid blastocysts|