Authors: Carrie Bedient, Ankita Raman, Forest Garner, Martha Aguirre, Chelsea Morrison, Shyni Thomas, Anika Bill, Bruce Shapiro
Objective: Assess relationships, if any, between embryo ploidy and the mean diameter of the follicle from which the oocyte originated.
Design: IRB-approved prospective observational cohort study.
Materials and Methods: Patients underwent routine ovarian stimulation with exogenous gonadotropins. During oocyte collection, follicle diameters were sonographically measured on two perpendicular axes, and the mean was calculated for each follicle.Embryos were group-cultured to the blastocyst stage according to mean follicle diameter (≤9.5, 10-12.5, 13-15.5, 16-18.5, 19-21.5, 22-24.5, 25-27.5, and ≥28mm). Next-generation sequencing (NGS) followed trophectodermbiopsy. Chi-square analysis compared aneuploidy rates among follicle diameter groups. Stepwise logistic regression was used to identify significant predictors of aneuploidy. P<0.05 was considered significant.
Results:There were 318 blastocysts derived from measured follicles that had definitive NGS results (normal or abnormal), while 4 embryos with no test result were excluded. Of the 318 included blastocysts, 169 had normal (euploid) copy numbers and 149 had abnormal (aneuploid) copy numbers.Chi-square analysis found no relationship between aneuploidy and follicle size group (P=0.46). Among variables available for logistic regression, only patient age was retained in the logistic model (P=0.0020), while mean follicle diameter and blastocyst morphology parameters (blastocyst expansion, ICM, and trophectoderm quality)were not significant.
Conclusions: Among blastocysts selected for biopsy, the diameter of the originating follicle was not a significant predictor of embryo ploidy.
Support: None
Mean follicle diameter | Blastocysts | Euploid blastocysts |
≤9.5mm | 12 | 5 (41.7%) |
10-12.5mm | 32 | 17 (53.1%) |
13-15.5mm | 51 | 29 (56.9%) |
16-18.5mm | 71 | 42 (59.2%) |
19-21.5mm | 75 | 39 (52.0%) |
22-24.5mm | 52 | 21 (40.4%) |
25-27.5mm | 15 | 10 (66.7%) |
≥28mm | 10 | 6 (60.0%) |
Total | 318 | 169 (53.1%) |